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Effect of Yiqi Jianpi Fang on Aberrant Crypt Foci Production in Carcinogenesis Wistar Rat Colorectal Cancer Model and Investigation on the Related Mechanism / 现代生物医学进展
Progress in Modern Biomedicine ; (24): 5043-5047, 2017.
Article in Chinese | WPRIM | ID: wpr-615397
ABSTRACT

Objective:

To investigate the inhibitory effect and the related mechanism of Yiqi Jianpi Fang on aberrant crypt focus (ACF) in Wister rat colorectal cancer model.

Methods:

50 Wistar rats were randomly divided into 5 groupsTCM low dose group(TCM solution diluted 3 times,5 mL/kg daily gastric volume),TCM middle dose group (5 mL/kg daily gastric volume),TCM high dose group (15 mL/kg daily gastric volume),model group,normal group.With 10 rats in each group.The colorectal tissues were observed under microscope after methylene blue staining by immunohistochemistry method.

Results:

Compared with the model group,the number of ACF and large ACF in each TCM group were decreased (P<0.05),and the number of ACF in the TCM middle dose group reduced most obvious,and the difference was statistically significant (P<0.05).The proliferation indexs of PCNA in intestinal gland cells 24 h and 48 h after modeling in the TCM groups were higher than those in the model group,and the difference was statistically significant(P<0.05).The apoptosis index of intestinal gland cells 24 h and 48 h after modeling in the TCM groups were higher than those in the model group,and the difference was statistically significant(P<0.05).The ectopic expression of β-catenin in TCM groups were lower than that in the model group,and it was highest in the high dose group,than was the low dose group,and the middle group was lowest(P<0.05).The expression of MMP-7 in TCM groups were lower than that in the model group,and it was highest in the low dose group,than was the high dose group,and the middle dose group was lowest (P<0.05).

Conclusion:

Yiqi Jianpi Fang can significantly reduce the number of ACF in Wister rats,inhibit the activation of Wnt signaling in colorectal cancer,reduce the incidence of colorectal cancer,and have a certain preventive effect.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Progress in Modern Biomedicine Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Progress in Modern Biomedicine Year: 2017 Type: Article