Study on main components of water extract in Xiefei-Pingchuanling using UPLC-MS / 国际中医中药杂志
International Journal of Traditional Chinese Medicine
;
(6): 530-534, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-616178
ABSTRACT
Objective To establish a Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS) chromatographic method for Xiefei-Pingchuanling extract, and to analyze the main components of its water extract. Methods The UPLC-MS was adopted with acetonitrile -0.2% formic acid aqueous solution (gradient elution) as mobile phase at a flow rate of 0.8 ml/min, detection wave length at 254 nm, and column temperature was 30 ℃. Results Eighteen components were separated from Xiefei-Pingchuanling extract, and eight ingredients were identified, such as the ephedrine, pseudoephedrine hydrochloride, salvianolic acidB, rhein, aloeemodin, chrysophanol, physcionwere. Conclusions The UPLC-MS chromatographic method has the specialty of stability and repeatability, and it is suitable for analysis of Xiefei-Pingchuanling extract.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
International Journal of Traditional Chinese Medicine
Year:
2017
Type:
Article
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