Relationship between Methylation of MGMT Gene and Protein Expression of DNMT1 in Gastric Cancer / 胃肠病学
Chinese Journal of Gastroenterology
;
(12): 407-410, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-616385
ABSTRACT
Background:
Studies have shown that promoter methylation of MGMT gene is closely related to many malignant tumor including gastric cancer.DNA methyltransferase 1 (DNMT1) is highly expressed in many malignant tumor tissues.However, studies on relationship between methylation of MGMT gene and DNMT1 expression in gastric cancer are rare.Aims:
To investigate the relationship between methylation of MGMT gene, protein expression of DNMT1 and gastric cancer.Methods:
Promoter methylation status of MGMT gene in 60 gastric adenocarcinoma tissues and corresponding paracancerous tissues were detected by methylation-specific PCR (MSP).RT-PCR and immunohistochemistry were used to measure mRNA and protein expressions of MGMT and DNMT1, respectively.Results:
Methylation rate of MGMT gene promoter in gastric cancer was significantly higher than that in paracancerous tissue (45.0% vs.13.3%, P<0.001).The positivity rate of MGMT mRNA in gastric cancer was significantly lower than that in paracancerous tissue (41.7% vs.93.3%, P<0.001), while the positivity rate of DNMT1 mRNA expression in gastric cancer was significantly higher than that in paracancerous tissue (76.7% vs.18.3%, P<0.001).Methylation rate of MGMT promoter in MGMT mRNA-negative expressed gastric cancer tissue was significantly higher than that in MGMT mRNA-positive expressed gastric cancer tissue (57.1% vs.28.0%, P<0.05).It showed a negative correlation between MGMT protein expression and DNMT1 protein expression in gastric cancer tissue (r=-0.795, P<0.01).Conclusions:
Promoter methylation of MGMT gene and high expression of DNMT1 may be associated with the development and progression of gastric cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Gastroenterology
Year:
2017
Type:
Article
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