Molecular mechanism of exercise intervention in diabetic cardiomyopathy in rats / 中国病理生理杂志

ABSTRACT

AIM: To investigate the effect of streptozotocin-induced diabetes on the expression of NADPH oxidase, and to determine the effect of exercise on the expression of NADPH oxidase.METHODS: One weeks after successful modeling, the effect of diabetes mellitus on the expression of NADPH oxidase subunits in the cardiac tissue was determined.The effect of exercise for 8 weeks on the protein expression of NADPH oxidase subunits and the effect of diabetes on the protein expression of collagen in the heart were observed, and whether 8 weeks of exercise affected the expression of collagen were also measured.RESULTS: Diabetes mellitus resulted in the increased expression of NADPH oxidase subunits p47phox and gp91phox in the cardiac ventricle, and exercise for 8 weeks inhibited the increase in the expression of NADPH oxidase subunits p47phox and gp91phox.Diabetes mellitus significantly increased the expression of p47phox in the atrial muscle, while exercise inhibited this increase.Diabetes mellitus significantly increased the levels of cardiac collagen III, while exercise reduced the increase in collagen protein.CONCLUSION: Reduction of diabetic rat heart p47phox and gp91phox expression by effective exercise therapy may be an important mechanism of improving the cardiac matrix by inhibiting myocardial oxidative stress and decreasing collagen expression, thus preventing diabetic cardiomyopathy.