Accumulation differences of four polyphyllins in Paris polyphylla and Paris polyphylla var.yunnanensis / 中成药

ABSTRACT

AIM To investigate the accumulation differences of polyphyllins Ⅰ,Ⅱ,Ⅵ and Ⅶ in Paris polyphylla Smith var.polyphylla and Paris polyphylla Smith var.yunnanensis (Franch.) Hand.-Mazz..METHODS The contents of four polyphyllins in the roots,leaves and stems of P.polyphylla were determined by HPLC.The fingerprints of twenty batches of samples were established.Cluster analysis was adopted in the evaluation of accumulation differences of various polyphyllins in different parts of two kinds of plants.RESULTS There were significant differences in various polyphyllins' contents and total contents in different populations of P.polyphylla (P ＜ 0.05,P ＜ 0.01).Polyphyllin Ⅵ was not detected in the roots,and the average contents of polyphyllins Ⅰ and Ⅱ were the highest.The polyphyllin Ⅶ' s average content and total content were the highest in the leaves.Only polyphyllin Ⅶ was detected in the stems,and the total content was the lowest.The similarities of roots of P.polyphylla and P.polyphylla var.yunnanensis were high,while those of leaves and stems were low.According to the total contents of polyphyllins in different parts,the samples could be divided into three types.CONCLUSION P.polyphylla can be considered as the substitute of P.polyphylla var.yunnanensis.