Bone marrow mesenchymal stem cell transplantation combined with Shenghuazhixue decoction in the treatment of postpartum subinvolution of uterus / 中国组织工程研究

ABSTRACT

BACKGROUND:Bone marrow mesenchymal stem cell (BMSCs) transplantation has been reported to promote the repair of uterine injury;Shenghuazhixue decoction functions to promote blood circulation and remove blood stasis, and dysmenorrhea. OBJECTIVE:To investigate the effect of BMSCs transplantation combined withShenghuazhixue decoction on subinvolution of uterus effect and the underlying mechanism. METHODS:The model of subinvolution of uterus was established in 40 femaleSprague-Dawley rats, followed by randomly divided into four groups control,Shenghuazhixue decoction, BMSCs, and combination groups. The control group received no treatment; at the 1st day after modeling, 1×107/L BMSCs (1 mL) were injected into the rat myometrium in the BMSCs and combination groups before laparotomy; the rats in theShenghuazhixue decoction group received no treatment after incision of uterus, and then rinsed using normal saline before closing the abdomen; theShenghuazhixue decoction and combination groups were given 8 mL/(kg?d)Shenghuazhixue detectionvia gavage at the 1st day after modeling, once daily, for consecutive 7 days. Afterwards, the relevant indexes were detected. RESULTS AND CONCLUSION:Compared with the control group, the body weight, uterine wet weight and uterine index in the combined group were significantly decreased after treatment (P < 0.05). The body weight and uterine wet weight showed significant differences betweenShenghuazhixue decoction and control groups (P < 0.05). Compared with the control group, the expression levels of intrerleukin-1 and tumor necrosis factor-α were significantly decreased, and the serum level of thromboxane B2 was significantly increased in theShenghuazhixue decoction group after treatment (P < 0.05). After treatment, the expression levels of intrerleukin-1 and tumor necrosis factor-α were significantly decreased, and the level of vascular endothelial growth factor was significantly increased in the BMSCs group compared with the control group (P < 0.05). Compared with the control group, there was significant decease in the expression levels of intrerleukin-1 and tumor necrosis factor-α, and significant increase in serum level of thromboxane B2 and vascular endothelial growth factor in the combination group (P < 0.01). These results suggest that BMSCs transplantation combined withShenghuazhixue detection can reduce postpartum body weight, uterine wet weight and index of uterus, and promote uterine involution in female rats, which may be through decreasing the levels of intrerleukin-1 and tumor necrosis factor-α as well as increasing the levels of thromboxane B2 and vascular endothelial growth factor.