Detection of serum vitamin D in T2DM patients with retinopathy and the relevance to oxidative stress / 中华内分泌外科杂志

ABSTRACT

Objective To detect the serum vitamin D levels in T2DM patients with retinopathy and to analyze the relevance to oxidative stress.Methods Totally 293 cases of T2DM were selected and they were divided into diabetic retinopathy (DR) group and non-diabetic retinopathy (NDR) group according to the results of fundus examination whether with or without DR.46 healthy volunteers were selected as control group (NC).The clinical information was collected,blood sugar such as fasting blood glucose (FBG),glycosylated hemoglobin (HbA1c),blood lipid index such as high-density lipoprotein cholesterol (HDL-C),total cholesterol (TC),triglyceride (TG),low density lipoprotein cholesterol (LDL-C),serum 25-hydroxyl vitamin D3 (25 (OH)D3),oxidative stress indicators such as malondialdehyde (MDA),superoxide dismutase (SOD) and reduced glutathione (GSH) were detected.The correlation between 25 (OH)D3 and clinical data,blood glucose,blood lipids,oxidative stress indicators was analyzed.Results 25(OH)D3 of the 3 groups had significantly difference based on statistical analysis (P＜ 0.05).In details,the 25(OH)D3 in DR and NDR group were significantly lower than that in NC group,and 25(OH) D3 in DR group was significantly lower than that in NDR group,and the differences had statistical significance (P＜0.05).MDA,SOD,and GSH had significantly difference between the 3 groups (P＜0.05).In details,MDA in DR group and NDR group were significantly higher than that in NC group.SOD and GSH were significantly lower than those in NC group,while MDA in DR group was significantly higher than that in NDR group.SOD and GSH were significantly lower than those in NDR group.The differences were statistically significant(P＜0.05).Relevant analysis showed that 25 (OH)D3 was significantly positively related to HDL-C,SOD and GSH in DR patients,and was significantly negatively related to disease course,FBG,and MDA.Multiple stepwise regression analysis showed that totally HDL-C (X1),SOD (X2),and MDA (X3)were included into the model.The regression equation was Y=15.434+0.261X 1 +0.078X2-0.121X3.Conclusion T2DM patients with retinopathy have the oxidative stress injury and their 25(OH)D3 declines,which may be involved in pathogenesis of DR by reciprocal causation.