Content Determination and Grey Relational Analysis of Cucurbitaceaes in Five Varieties of Cucurbitaceae Plants / 中国药师
China Pharmacist
;
(12): 1201-1205, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-617503
ABSTRACT
Objective:
To establish an HPLC method for the content determination of cucurbitacin B and E in cucurbitaceae plants, and evaluate the quality of cucurbits by grey relational analysis.Methods:
A Diamonsil-C18 column (250 mm×4.6 mm, 5 μm) was adopted and the UV detection wavelength was 234 nm at the flow rate of 1.0 ml·min-1.The mobile phase consisted of 0.1% acetic acid and acetonitrile with gradient elution, and the column temperature was 30℃.In addition,grey relational analysis was carried out for the comprehensive evaluation.Results:
The linear range of cucurbitacin B and E was 0.894-44.715 μg·ml-1(r=0.999 6) and 0.257-12.825 μg·ml-1(r=0.999 7),respectively.The average recovery was 98.8%-100.3% and 98.0%-100.1%(RSD<0.88%,n=6), respectively.According to the grey correlation analysis on the components, Cucumis melo L had the best quality, and Momordica cochinchinensis (Lour.) Spreng showed the worst quality.Conclusion:
The established method is rapid and convenient, which can be used for the content determination of cucurbitacin B and E in cucurbits, and the comprehensive quality evaluation of cucurbits.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2017
Type:
Article
Similar
MEDLINE
...
LILACS
LIS