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Study on UPLC Fingerprint of Gehua Formula Granules / 中国药房
China Pharmacy ; (12): 2978-2980, 2017.
Article in Chinese | WPRIM | ID: wpr-617680
ABSTRACT

OBJECTIVE:

To establish UPLC fingerprint of Gehua formula granules.

METHODS:

UPLC method were adopted. The determination was performed on Zorbax Eclipse XDB-C18 column with mobile phase consisted of acetonitrile-water at the flow rate of 0.5 mL/min. The detection wavelength was set at 264 nm,column temperature was 25 ℃,and the sample size was 1 μL. Using tectorigenin as reference substance,UPLC chromatograms of 10 batches of Gehua formula granules were determined. The common peak identification and similarity evaluation were conducted by TCM Chromatogram Fingerprint Similarity Evaluation Sys-tem(2004 A edition).

RESULTS:

14 common peaks were identified in UPLC chromatograms of 10 batches of Gehua formula gran-ules and similarities were all higher than 0.90. UPLC chromatograms of 10 batches of samples were in good agreement with control fingerprint.

CONCLUSIONS:

Established UPLC fingerprint can provide reference for identification and quality evaluation of Gehua formula granules.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2017 Type: Article