Identification of Genomic Aberrations by Array Comparative Genomic Hybridization in Patients with Aortic Dissections
The Korean Journal of Thoracic and Cardiovascular Surgery
;
: 123-130, 2011.
Article
in English
| WPRIM
| ID: wpr-61790
ABSTRACT
BACKGROUND:
The aim of the present study was to identify chromosomal loci that contribute to the pathogenesis of aortic dissection (AD) in a Korean population using array comparative genomic hybridization (CGH) and to confirm the results using real-time polymerase chain reaction (PCR). MATERIALS ANDMETHODS:
Eighteen patients with ADs were enrolled in this study. Genomic DNA was extracted from individual blood samples, and array CGH analyses were performed. Four corresponding genes with obvious genomic changes were analyzed using real-time PCR in order to assess the level of genomic imbalance identified by array CGH.RESULTS:
Genomic gains were most frequently detected at 8q24.3 (56%), followed by regions 7q35, 11q12.2, and 15q25.2 (50%). Genomic losses were most frequently observed at 4q35.2 (56%). Real-time PCR confirmed the results of the array CGH studies of the COL6A2, DGCR14, PCSK6, and SDHA genes.CONCLUSION:
This is the first study to identify candidate regions by array CGH in patients with ADs. The identification of genes that may predispose an individual to AD may lead to a better understanding of the mechanism of AD formation. Further multicenter studies comparing cohorts of patients of different ethnicities are warranted.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aorta
/
DNA
/
Polymerase Chain Reaction
/
Cohort Studies
/
Comparative Genomic Hybridization
/
Real-Time Polymerase Chain Reaction
Type of study:
Controlled clinical trial
/
Diagnostic study
/
Etiology study
/
Incidence study
/
Observational study
/
Risk factors
Limits:
Humans
Language:
English
Journal:
The Korean Journal of Thoracic and Cardiovascular Surgery
Year:
2011
Type:
Article
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