Generate and purify antibody against human Heparansulphate interacting protein (hHIp) in rabbit
Journal of Medical Research
;
: 1-5, 2007.
Article
in Vietnamese
| WPRIM
| ID: wpr-618
ABSTRACT
Background:
Human Heparansulphate interacting protein (hHip) has been shown to participate in biological processes of cells. Several studies indicated that hHip transcript is up regulated in several of cancer tissues including those of thyroid, colon, breast and prostate. Antibody against hHIP is necessary for methods to evaluate protein level of HIP in cancer tissues.Objectives:
The aims of study was to induce anti hHIP antibody in rabbit and purify and conserve purified anti hHIP antibody. Subjects andmethod:
The study included 9 adult and healthy rabbits with the weight 2 - 2.5kg. Immunization hHIP peptide-KLH in rabbit. Purify anti hHIP antibody using affinity chromatography.Results:
The results shown synthesize hHIP peptide and conjugate it with carrier protein. Sensitive rabbit better meet with hHIP-KLH antibody. The Ig concentration obtained in sensitive rabbit was rather high and equal. Immunization hHIP-KLH successfully in rabbit. Obtainment valuable amount of anti hHIP antibody.Conclusion:
Successfully induce and purify anti hHIP antibody from rabbit. Establish a standard protocol for polyclonal antibody against small peptide in rabbit.\r\n', u'\r\n', u'
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Carrier Proteins
/
Chemistry
/
Dosage
Limits:
Animals
Language:
Vietnamese
Journal:
Journal of Medical Research
Year:
2007
Type:
Article
Similar
MEDLINE
...
LILACS
LIS