Optimization of Extraction Technology of Polyphenols from Juglans regia Branch by Response Surface Method and Study on Its Antioxidant Activity in vitro / 中国药房

ABSTRACT

OBJECTIVE:To optimize the extraction technology of polyphenols from Juglans regia branch and evaluate its anti-oxidant activity in vitro. METHODS:Using extraction amount of polyphenols from J. regia branch as response value,solvent-solid ratio,extraction temperature and ethanol volume fraction as response factors,based on single factor test,response surface method was used to optimize the extraction technology of polyphenols from J. regia branch. Using vitamin C as positive control,scaveng-ing on hydroxyl radicals and 1,1-diphenyl-2-picrylhydrazyl(DPPH)radicals and total reducing activities of polyphenols from J. re-gia branch were investigated. And its antioxidant activity in vitro was evaluated. RESULTS:Optimized extraction conditions for polyphenols from J. regia branch was as follow as solid-liquid ratio of 125(g/mL),extraction temperature of 50 ℃,ethanol vol-ume fraction of 70%. The extraction amount of polyphenols from J. regia branch was 9.30 mg/g(RSD=0.57%,n=3)in the veri-fication test. Clearance rate on hydroxyl radicals and DPPH radicals and total reducing activity of polyphenols from J. regia branch were respectively 50.24%,95.42%,1.118 when it was under the mass concentration of 12.0,3.0,3.0μg/mL;and the related data of vitamin C was 93.71%,46.17%,0.628 under the same mass concentration(P<0.05). CONCLUSIONS:Extraction technology of polyphenols from J. regia branch optimized by response surface method is stable and feasible;polyphenols from J. regia branch shows certain antioxidant activity in vitro.