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Determination of Low-concentration Clenbuterol in Edible Meat Using High Performance Liquid Chromatography-tandem Mass Spectrometry / 中国运动医学杂志
Chinese Journal of Sports Medicine ; (6): 429-433, 2017.
Article in Chinese | WPRIM | ID: wpr-618400
ABSTRACT
Objective To determine low-concentration clenbuterol in edible meat based on the solidphase extraction coupled with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).Method The homogenated sample was acidized to remove proteins,and purified using the liquid-liquid extraction and MCX Oasis solid prepared extraction column,then further treated with gradient elution with the mobile phase of ammonium formate (10 mmol/L and pH 3.5) and acetonitrile.The clenbuterol was completely separated on Eclipse C18 (1.8 μm,4.6×100 mm) column and detected in multiple reaction monitoring (MRM) mode.Results A good linearity was achieved for clenbuterol in the arrange of 0.01~0.2 μg/kg based on the internal standard calibration of D9-clenbuterol,with the linearity correlation coefficient greater than 0.99 and the detection limit of 0.005 μg/kg.The relative recovery of target compounds spiked in blank sample at three levels ranging from 78.8 to 114.8%,with the relative standard deviations less than 10%.Conclusion The method in this research is simple,rapid,reliable and suitable to confirm low-concentration clenbuterol in edible meat.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Sports Medicine Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Sports Medicine Year: 2017 Type: Article