Two kinds of detection methods of antinuclear antibodies in diagnosis of systemic lupus erythematosus / 实用医学杂志

ABSTRACT

Objective To detect serum antinuclear antibody (ANA) with enzyme-linked immunosorbent (ELISA) and linear immunoblot technique (LIA) and to evaluate and compare the performance of two methods in auxiliary diagnosis of systemic lupus erythematosus (SLE). Methods ANA detected by both ELISA and LIA of 597 cases were collected in the last two years. The results were retrospective analyzed. The sensitivity, specificity, accuracy, positive predictive value, negative predictive value, and the consistency of the two methods were compared and analyzed. Results The sensitivity of ELISA and LIA was 79.3% and 86.6% respectively for SLE patients; specificity 81.7% and 77.3%; accuracy 81.4% and 78.6%, positive predictive value 40.9%, and 37.8%;negative predictive value 96.1% and 97.3% respectively. No significant difference was found between the two methods (P > 0.05). The results showed that the coincidence rate was 81.4%, and the Kappa test 0.55. The Kappa test of the two methods in SLE group was 0.403. Conclusion No statistical significance is found in the comparison of ELISA method and LIA method to detect ANA for the diagnosis of SLE and the combination of two kinds of detection method has important application value.