Correlation between CEUS parameters and angiogenesis and cell proliferation of gallbladder carcinoma transplant tumor in nude mice / 中国医学影像技术

ABSTRACT

Objective To explore the correlation between CEUS blood perfusion characteristics and angiogenesis and cell proliferation in nude mice models of gallbladder carcinoma transplant tumor.Methods Nude mice models of gallbladder carcinoma transplant tumor were established.The blood perfusion characteristics of the nude mice models were quantitatively analyzed,CEUS quantitative parameters and the expression levels of microvessel density (MVD),vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) immunohistochemically were obtained.The correlations were performed.Results Twenty tumors were successfully established.The CEUS of 16 tumors was successful.There was positive correlation between peak intensity (PI) and MVD,PCNA (r=0.635,0.514,P=0.008,0.042).The CEUS parameters were not correlated with expression of VEGF.MVD had positive correlation with expression of VEGF,PCNA (r=0.680,0.650,P=0.004,0.006);VEGF had positive correlation with expression of PCNA (r=0.611,P=0.012).Conclusion The PI of CEUS is positive correlation with MVD and PCNA in nude mice models of gallbladder carcinoma transplant tumor,which may be helpful to evaluate tumor angiogenesis and cell proliferation state.The MVD,VEGF and PCNA positively correlates to each other,which indicates there is the relationship of reciprocal promotion between tumor angiogenesis and cell proliferation,and is good for tumor growth.