Altered Regulation of 11beta-hydroxysteroid Dehydrogenase II in the Kidney of Rats with Experimental Hypertension
Electrolytes & Blood Pressure
; : 89-94, 2007.
Article
in En
| WPRIM
| ID: wpr-62075
Responsible library:
WPRO
ABSTRACT
The present study was aimed at investigating the role of type II 11beta-hydroxysteroid dehydrogenase (IIbeta- HSD II) in the development of hypertension. Two-kidney, one-clip (2K1C), deoxycorticosterone acetate (DOCA)/salt, or NG-nitro-L-arginine methyl ester (L-NAME) hypertension was induced in male Sprague- Dawley rats. Four weeks later, the expression of 11beta-HSD II mRNA was determined in the kidney by Northern blot analysis. The plasma level of aldosterone was measured by radioimmunoassay. In 2K1C hypertension, the expression of 11beta-HSD II was decreased in the clipped kidney and increased in the non-clipped kidney. The expression was increased in the remnant kidney of DOCA/salt hypertension, while decreased in the kidneys of L-NAME hypertension. The plasma level of aldosterone was increased, decreased, and remained unchanged in 2K1C, DOCA/salt, and L-NAME hypertension, respectively. The down-regulation of 11beta-HSD II may contribute to the sodium retention, thereby increasing the blood pressure in 2K1C and L-NAME hypertension. On the contrary, the up-regulation in DOCA/salt hypertension may play a compensatory role to dissipate the sodium retention.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Plasma
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Sodium
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Blood Pressure
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RNA, Messenger
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Radioimmunoassay
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Down-Regulation
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Up-Regulation
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Blotting, Northern
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NG-Nitroarginine Methyl Ester
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11-beta-Hydroxysteroid Dehydrogenases
Limits:
Animals
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Humans
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Male
Language:
En
Journal:
Electrolytes & Blood Pressure
Year:
2007
Type:
Article