Change of Plasma Xanthine Oxidase Activity by Intermittent Hepatic Ischemia-Reperfusion / 대한마취과학회지
Korean Journal of Anesthesiology
;
: 692-697, 2004.
Article
in Korean
| WPRIM
| ID: wpr-62093
ABSTRACT
BACKGROUND:
The pringle maneuver (PM), hepatic inflow occlusion, during hepatic surgery reduces intraoperative bleeding and blood transfusion requirement, but hepatic ischemia/reperfusion injury is inevitable. During ischemia, xanthine oxidoreductase is converted to xanthine oxidase (XO), which can serve as a critical source of reactive oxygen species (reduces O2 to O2 .-) that contribute to inflammatory signaling, ischemia-reperfusion injury, and an impaired vascular function. The purpose of the present study was to follow changes of XO activity and O2 .- production during hepatic surgery under PM.METHODS:
Eleven patients that underwent hepatectomy under intermittent PM were studied. Blood was withdrawn before PM, and 10 and 20 minutes after final reperfusion. Plasma XO activity was measured using a spectrophotometer after incubating plasma with/without xanthine for one-hour. Superoxide (O2 -) production was followed by measuring by cytochrome c reduction by plasma XO.RESULTS:
After final reperfusion, plasma XO activity had increased four-fold (0.36 +/- 0.06 to 1.25 +/- 0.25 mU/ml) with a concomitant increase in O2 .- production (0.66 +/- 0.29 to 1.66 +/- 0.40microM/min).CONCLUSIONS:
Significantly more XO is released into the systemic circulation after intermittent PM, with subsequently increased O2 .- production. The significant contribution of XO to hepatic surgery under PM might be beneficially managed using an anesthetic with a known antioxidative effect.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasma
/
Xanthine Dehydrogenase
/
Xanthine Oxidase
/
Blood Transfusion
/
Reperfusion
/
Reperfusion Injury
/
Reactive Oxygen Species
/
Superoxides
/
Xanthine
/
Cytochromes c
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Anesthesiology
Year:
2004
Type:
Article
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