A high activity restriction enzyme recognizing GCGC

ABSTRACT

A study was performed to identify a high activity restriction enzyme recognizing 5’-GCGC-3’. Isoschizomer Hhal is a endonucleaza, which has been found in a bacteria isolated from a soil sample picked up in Nghean province. This enzyme has a similar activity with Hha l but it is a other protein of other host bacteria, therefore, it is temporary called a isoschizomer of Hhal. About the enzyme’s specific, Hhal cleaves 5 standard DNA molecules at 384 sites, in which 215 sites at DNA , 103 sites at DNA T7, 18 sites at x174, 31 sites at pBR 322 and 17 sites at pUC 19. Isoschizomer Hhal with high activity, stable met the need of users and laboratory of Vietnam.