Protection of INS-1 cells from STZ injury by gastrodine / 中华内分泌外科杂志

ABSTRACT

ObjectiveTo study effects of gastrodine (GAS) on insulinoma (INS-1) cells and the protection of INS-1 ceils from steptozotocin (STZ) injury by gaatrodine. MethodsThe experiment was carried out in 5 groups normal control group ( NC), GAS group (GAS), streptozotocin group (STZ), GAS protection group ( GAS +STZ) and GAS repair group (STZ +GAS). INS-1 cells were cultured, the cell viability was determined by tetrazolium (MTT) assay, insulin concentration was detected by radioimmunoassay, and malondialdehyde (MDA)concentration and total antioxidant capacity (T-AOC) of the culture medium were measured by colorimetry. Results GAS promoted insulin release of INS-1 cells (P＜0.05, P＜O.01). Low-concentration GAS could increase viability of INS-1 cells ( P ＜ 0.01 ). GAS could increase viability of the injured INS-1 cells (P ＜ 0.01 ). High concentration GAS contributed in repair of INS-1 cells injured by STZ and promoted insulin serection ( P ＜ 0.01 ). GAScould decrease MDA concentration (P ＜0.01 ) and significantly increase T-AOC capacity of INS-1 cells injured by STZ (P ＜0.01 ). ConclusionsGAS can increase INS-1 viability, promote insulin secretion of INS-1 cells, alleviate INS-1 cells injury caused by STZ, and strengthen the antioxidant capacity of INS-1 cells injured by STZ.