Construction of human Egr-1 promoter and its response to ionizing radiation in tumor cells / 细胞与分子免疫学杂志
Chinese Journal of Cellular and Molecular Immunology
;
(12): 973-975, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-621945
ABSTRACT
AIM:
To construct human Egr-1 promoter luciferase reporter system and study its activity induced by i-onizing radiation.METHODS:
Egr-1 promoter was obtained by human genomic PCR and cloned into pGL3-basic vector. After transfection of recombinant plasmid into human tumor cells, the Egr-1 promoter activity induced by ionizing radiation was detected by luciferase reporter assay.RESULTS:
The luciferasy reporter system of Egr-1 promoter was successfully constructed. The activity of Egr-1 promoter was substantially increased after different doses of IR and reached to the peak at the time point of 48h after IR.CONCLUSION:
The Egr-1 promoter was constructed in this study showed IR inducible activity in tumor cells, laying foundation for the research of radiation, mediated gene therapy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Cellular and Molecular Immunology
Year:
2009
Type:
Article
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