Screening and identification of anti-HRPII single chain Fv antibodies of Plasmodium falciparum / 细胞与分子免疫学杂志
Chinese Journal of Cellular and Molecular Immunology
;
(12): 185-188, 2000.
Article
in Chinese
| WPRIM
| ID: wpr-622103
ABSTRACT
To develop simple, rapid, and efficacious diagnostic methods for malaria is one of the remaining key tasks for malaria control. Previously, we have created a phage-displayed antibody library against Plasmodium falciparum. Six clones of antibody with good reactivity to HRP-II in ELISA were isolated from the library after 3 rounds of enrichment. Soluble ScFvs were produced and the characteristics were determined. The results of Western blot showed that they could bind to HRP-II specifically and had a relative molecular mass(Mr) about 31 000. The work provided a solid fund for diagnostic kit development for malaria.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Language:
Chinese
Journal:
Chinese Journal of Cellular and Molecular Immunology
Year:
2000
Type:
Article
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