Expression of truncated fragment of human OPG in CHO-DHFR- cells and its bioactivity characterization / 细胞与分子免疫学杂志
Chinese Journal of Cellular and Molecular Immunology
;
(12): 1002-1004, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-622219
ABSTRACT
AIM:
To obtain high level expression of recombinant human truncated osteoprotegerin (TOPG) with higher bioactivity in CHO-DHFR~-cells.METHODS:
The recombinant vector pcDNA3.1/DHFR-TOPG was constructed and transfected into CHO-DHFR~- Cells by the directions of LipofectAMINE~(TM)2000 for stable expression. The stable expression cell strains were screened by selective medium IMDM with 50 mL/L FCS, then serially passed in methotraxate (MTX) for gene amplification. The expression were analyzed by ELISA and RT-PCR. At last, the bioactivity analysis was performed in vitro.RESULTS:
The expression level of recombinant truncated human OPG was up to 6 mg/L·72 h, and it had significant suppression effect on the formation of OLC(P<0.05).CONCLUSION:
Recombinant truncated human OPG has high expression and bioactivity. The results make it possible for further studying and clinical implying of OPG.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Cellular and Molecular Immunology
Year:
2009
Type:
Article
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