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The purification of factor B in human sera by liquid chromatography / 细胞与分子免疫学杂志
Chinese Journal of Cellular and Molecular Immunology ; (12): 82-83, 2001.
Article in Chinese | WPRIM | ID: wpr-622252
ABSTRACT
Aim To establish a method to purify factor B in human sera. Methods A combination of euglobulin precipitation, ion-exchange chromatography,(NH4)2SO4 precipitation and affinity chromatography was used in the process of purification. Results Final product of 118.75 mg/L plasma factor B was obtained. By SDS-PAGE, thin layer scanner and activity assay,the purity reached 95% , specific activity was 1.91× 109 IU/g, and the activity yield was 59.28% . Conclusion This simple method with high yield can be used for laboratory research and large-scale preparation.
Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Cellular and Molecular Immunology Year: 2001 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Cellular and Molecular Immunology Year: 2001 Type: Article