The correlation of Galectin-3 gene polymorphism and breast cancer / 中华内分泌外科杂志

ABSTRACT

Objective To investigate the association of Galectin-3 ( lectin, galactoside-binding soluble , LGALS3)gene-191(rs4644)polymorphism and breast cancer in Chinese female population .Methods Galectin-3 gene rs4644 polymorphisms was genotyped by polymerase chain reaction-restriction fragment length polymor-phism(PCR-RFLP)in 172 breast cancer patients and 17 controls.The experimental results of PCR-RFLP were verified and sample of 10 cases were sequenced randomly .Results The frequency of CC ,CA and AA genotype in breast cancer group and normal control group was 72.7%, 25.0%, 2.3% and 52.9%, 41.2%, 5.9%, respectively .The frequency of C and A allele in breast cancer group and normal control group was 85.2%, 14.8%and 73.5%, 26.5% respectively .The genotype and frequency of allele had no statistical difference between breast cancer patients and the control group .rs4644C allele was more likely to occur in breast cancer patients with T>2 cm (χ2 =8.295,P=0.004) and Ki67<14%(χ2 =3.917,P=0.048).Conclusions There is no significant association between Galectin-3 gene 191 polymorphism rs4644 and susceptibility of breast cancer in Chinese female population .It is closely associated with the size of tumor and Ki 67.