The determination heparansulphat interacing protein (HIP) transcript in cancer tissues using semiquantitative PCR and quantitative PCR methods

ABSTRACT

Background: Semiquantitative PCR and quantitative PCR are accurate and simple methods. They are commonly used to determine amplified gene levels in PCR reaction. Objective: Using semiquantitative PCR and quantitative PCR methods to determine HIP transcript levels in cancer and normal tissue; to evaluate sensibility of two methods. Subject and methods: 30 cancer patients were diagnosed based on clinical, para-clinical (ultrasound, biochemistry, histopathology) at K hospital in Hanoi. 15 benign tissue samples are used for control.mRNA was extracted from cancer and normal tissues, cDNA was synthesized by reverse transcript-polymerase chain reaction (RT - PCR); HIP transcript was determined using semiquantitative PCR and quantitative PCR methods. Results: Both methods showed the same results: HIP transcript was increase in cancer tissues but very low in normal tissues. This showed that HIP was linked closely with the development of cancer tissue. Conclusions: Levels of HIP transcript was different between cancer tissue and the normal control. Semi- quantitative PCR and quantitative PCR are useful methods to determine HIP transcript for cancer diagnosis. \r\n', u'\r\n', u'\r\n', u'