The effect of granulocyte-macrophage-stimulating factor on the gene expression in human trophoblast of early pregnancy / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
;
: 905-914, 2010.
Article
in Korean
| WPRIM
| ID: wpr-62444
ABSTRACT
OBJECTIVE:
Granulocyte-macrophage colony-stimulating factor (GM-CSF) facilitates mammalian embryonic development and implantation. However, its biological function after implantation is not elucidated. The aim of this study is to assess the changes of gene expression by GM-CSF in human trophoblast obtained in early pregnancy.METHODS:
Human trophoblast obtained in early pregnancy was cultured with or without GM-CSF. The difference of gene expression was evaluated with microarray and selected genes were reevaluated with real-time reverse transcription-polymerase chain reaction (RT-PCR).RESULTS:
Microarray analysis revealed that the expressions of 468 genes were increased while those of 40 genes were decreased by GM-CSF. These genes were evaluated according to the known biologic pathways. The regulation of actin cytoskeleton and focal adhesion pathways were mostly influenced by GM-CSF. Annexin A2, thymosin-like 3, vimentin, myogenin, ACK1, and tensin1 genes were selected for real-time RT-PCR. The increased expressions of of vimentin and ACK1, and decreased expressions of tensin1 were confirmed by real-time RT-PCR.CONCLUSION:
GM-CSF activates focal adhesion pathway in human trophoblast by increasing the expression of vimentin and ACK1, and decreasing the expression of tensin1.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Trophoblasts
/
Vimentin
/
Actin Cytoskeleton
/
Gene Expression
/
Granulocyte-Macrophage Colony-Stimulating Factor
/
Annexin A2
/
Myogenin
/
Focal Adhesions
/
Microarray Analysis
/
Embryonic Development
Limits:
Female
/
Humans
/
Pregnancy
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2010
Type:
Article
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