Your browser doesn't support javascript.
loading
The effect of granulocyte-macrophage-stimulating factor on the gene expression in human trophoblast of early pregnancy / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology ; : 905-914, 2010.
Article in Korean | WPRIM | ID: wpr-62444
ABSTRACT

OBJECTIVE:

Granulocyte-macrophage colony-stimulating factor (GM-CSF) facilitates mammalian embryonic development and implantation. However, its biological function after implantation is not elucidated. The aim of this study is to assess the changes of gene expression by GM-CSF in human trophoblast obtained in early pregnancy.

METHODS:

Human trophoblast obtained in early pregnancy was cultured with or without GM-CSF. The difference of gene expression was evaluated with microarray and selected genes were reevaluated with real-time reverse transcription-polymerase chain reaction (RT-PCR).

RESULTS:

Microarray analysis revealed that the expressions of 468 genes were increased while those of 40 genes were decreased by GM-CSF. These genes were evaluated according to the known biologic pathways. The regulation of actin cytoskeleton and focal adhesion pathways were mostly influenced by GM-CSF. Annexin A2, thymosin-like 3, vimentin, myogenin, ACK1, and tensin1 genes were selected for real-time RT-PCR. The increased expressions of of vimentin and ACK1, and decreased expressions of tensin1 were confirmed by real-time RT-PCR.

CONCLUSION:

GM-CSF activates focal adhesion pathway in human trophoblast by increasing the expression of vimentin and ACK1, and decreasing the expression of tensin1.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Trophoblasts / Vimentin / Actin Cytoskeleton / Gene Expression / Granulocyte-Macrophage Colony-Stimulating Factor / Annexin A2 / Myogenin / Focal Adhesions / Microarray Analysis / Embryonic Development Limits: Female / Humans / Pregnancy Language: Korean Journal: Korean Journal of Obstetrics and Gynecology Year: 2010 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Trophoblasts / Vimentin / Actin Cytoskeleton / Gene Expression / Granulocyte-Macrophage Colony-Stimulating Factor / Annexin A2 / Myogenin / Focal Adhesions / Microarray Analysis / Embryonic Development Limits: Female / Humans / Pregnancy Language: Korean Journal: Korean Journal of Obstetrics and Gynecology Year: 2010 Type: Article