Development of ochratoxin A detection based on electrochemical sensor using Au-ball labels
Malaysian Journal of Microbiology
;
: 339-344, 2016.
Article
in English
| WPRIM
| ID: wpr-626888
ABSTRACT
Aims:
The present study was aimed to develop a highly sensitive and rapid method for ochratoxin A (OTA) detection. Methodology andresults:
In this study, an electrochemical peptidesensor for OTA detection was developed using numerous number of Au particles coated on the surface of silica particle (Au-ball). Moreover, this assay was performed in 384 well plate, so the multiple detections was done. The synthesized silica particle was spherical in shape and size was 275±17 nm. After coated with Au layer, size of Au-ball was 280±14 nm. Au particles loaded can be taken up resulting in approx. 1×107 Au3+ molecules per silica particle. Moreover, the optimization conditions were studied. The limit of detection of this assay showed as low as 2 ppb. Conclusion, significance and impact study This platform showed rapid, sensitive and specific to ochratoxin A detection. In spite of these advantages, this Au-ball based peptidesensors is suitable to use in the detection of ochratoxin A contaminated in coffee or seed industry.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
English
Journal:
Malaysian Journal of Microbiology
Year:
2016
Type:
Article
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