Ability of Acanthamoeba Cyst to Excyst at Different Temperatures / Jurnal Sains Kesihatan Malaysia
Malaysian Journal of Health Sciences
;
: 41-43, 2011.
Article
in English
| WPRIM
| ID: wpr-629187
ABSTRACT
This study was carried out to observe thermotolerance ability of Acanthamoeba spp. A total of 32 Acanthamoeba spp. isolates obtained from water taps, sinks, swimming pools and sea water were used. Trophozoites of Acanthamoeba spp. were inoculated onto non-nutrient agar (NNA) seeded with heat-killed Escherichia coli using aseptic technique and incubated for 14 days at 30°C to obtain the cyst. The cysts were subcultured onto new agar plates for thermotolerance test at 37°C and 42°C. The plates were observed until 96 hours after incubation for excystation of Acanthamoeba before being declared negative. Overall, 81.25% of samples were able to excyst at 37°C while 37.5% were able to excyst at 42°C. Thermotolerant Acanthamoeba is associated with high pathogenicity potential.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
English
Journal:
Malaysian Journal of Health Sciences
Year:
2011
Type:
Article
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