Negative cross-reactivity of rabbit anti-Malassezia furfur antibodies with other yeasts
The Malaysian Journal of Pathology
;
: 123-5, 2005.
Article
in Malayalam
| WPRIM
| ID: wpr-629304
ABSTRACT
Anti-Malassezia furfur monospecific polyclonal antibodies was produced by repeated immunization of rabbit with Malassezia furfur yeast cells mixed with Freud adjuvant. The antibody titres of respective rabbit's serum samples prior to and after each immunization against M. furfur were assayed by indirect immunofluorescence technique using the M. furfur whole yeast antigen fixed in Teflon coated slides. The highest anti-M. furfur antibody titre achieved was 1 in 1280 dilution. At 120 dilution, none of the respective serum samples taken at various stages of immunization gave positive immunofluorescent staining against any of the other species of yeasts tested in this study. Anti-M. furfur monospecific polyclonal antibodies produced in rabbit in this study has the potential for diagnostic application in immunohistochemical detection of M. furfur in human tissues.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Immunization
/
Malassezia
/
Antibodies
Limits:
Animals
Language:
Malayalam
Journal:
The Malaysian Journal of Pathology
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS