Strategy for generating tissue-engineered human bone construct
The Medical Journal of Malaysia
;
: 43-44, 2004.
Article
in Malayalam
| WPRIM
| ID: wpr-629925
ABSTRACT
The strategy used to generate tissue-engineered bone construct, in view of future clinical application is presented here. Osteoprogenitor cells from periosteum of consenting scoliosis patients were isolated. Growth factors viz TGF-B2, bFGF and IGF-1 were used in concert to increase cell proliferation during in vitro cell expansion. Porous tricalcium phosphate (TCP)-hydroxyapatite (HA) scaffold was used as the scaffold to form 3D bone construct. We found that the addition of growth factors, greatly increased cell growth by 2 to 7 fold. TCP/HA proved to be the ideal scaffold for cell attachment and proliferation. Hence, this model will be further carried out on animal trial.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Culture Techniques
/
Periosteum
/
Bone Regeneration
/
Cell Division
/
Collagen
/
Bone Transplantation
/
Tissue Engineering
/
Mesenchymal Stem Cell Transplantation
Type of study:
Prognostic study
Language:
Malayalam
Journal:
The Medical Journal of Malaysia
Year:
2004
Type:
Article
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