Cell death induced by hydroxyapatite on L929 fibroblast cells
The Medical Journal of Malaysia
; : 176-177, 2004.
Article
in Ml
| WPRIM
| ID: wpr-629952
Responsible library:
WPRO
ABSTRACT
Biomaterials intended for end-use application as bone-graft substitutes have to undergo safety evaluation. In this study, we investigated the in vitro cytotoxic effects especially to determine the mode of death of two hydroxyapatite compounds (HA2, HA3) which were synthesized locally. The methods used for cytotoxicity was the standard MTT assay whereas AO/PI staining was performed to determine the mode of cell death in HA treated L929 fibroblasts. Our results demonstrated that both HA2 and HA3 were not significantly cytotoxic as more than 75% cells after 72 hours treatment were viable. Furthermore, we found that the major mode of cell death in HA treated cells was apoptosis. In conclusion, our results demonstrated that these hydroxyapatite compounds are not cytotoxic where the mode of death was primarily via apoptosis.
Full text:
1
Index:
WPRIM
Main subject:
Prostheses and Implants
/
Biocompatible Materials
/
Cell Death
/
Apoptosis
/
Durapatite
/
Bone Substitutes
/
L Cells
Language:
Ml
Journal:
The Medical Journal of Malaysia
Year:
2004
Type:
Article