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Gene expression characteristic in human auricular cartilage tissue engineering
The Medical Journal of Malaysia ; : 190-191, 2004.
Article in Malayalam | WPRIM | ID: wpr-629960
ABSTRACT
This study was to assess collagen type II and collagen type I gene expression in tissue-engineered human auricular cartilage formed via tissue engineering technique. Large-scale culture expansions were transformed into 3D in vitro construct and were implanted subcutaneously on the dorsal of athymic mice. After 8 weeks, explanted construct was processed in the same manner of native cartilage to facilitate cells for gene expression analysis. Isolated cells from in vivo construct demonstrated expression of type II collagen gene comparable to native cartilage. This study verified that tissue-engineered auricular cartilage expressed cartilage specific gene, collagen type II after in vivo maturation.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Phenotype / Cartilage / Gene Expression / Cells, Cultured / Actins / Cellular Senescence / Chondrocytes / Reverse Transcriptase Polymerase Chain Reaction / Collagen Type I / Collagen Type II Language: Malayalam Journal: The Medical Journal of Malaysia Year: 2004 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phenotype / Cartilage / Gene Expression / Cells, Cultured / Actins / Cellular Senescence / Chondrocytes / Reverse Transcriptase Polymerase Chain Reaction / Collagen Type I / Collagen Type II Language: Malayalam Journal: The Medical Journal of Malaysia Year: 2004 Type: Article