The stemness gene expression of cultured human amniotic epithelial cells in serial passages
The Medical Journal of Malaysia
;
: 53-54, 2008.
Article
in Malayalam
| WPRIM
| ID: wpr-629976
ABSTRACT
The aim of the study is to evaluate the stemness gene expression of cultured human amniotic epithelial cells (HAECs) in serial passages. HAECs obtained from human term placentae were cultured in F12DMEM(11) + 10% FBS +10ng/ml EGF in serial passages (P0, P1, P2 and P4). Quantitative RT-PCR was used to assess the gene expression analysis. The results showed that cultured HAECs expressed and downregulated the stemness genes expression for Oct-4, Sox-2, Nanog3, FGF4, Rex-1, FZD-9, BST-1 ABCG2. However, vimentin and nestin gene expression were upregulated. The results suggested that cultured HAECs may have pluripotent and multipotent properties.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Malayalam
Journal:
The Medical Journal of Malaysia
Year:
2008
Type:
Article
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