HPLC DETERMINATION OF GLYCYRRHIZIC ACID TABLETAE FROM HERBAL / Монголын эм зүй, эм судлал

ABSTRACT

Introduction: It is very important to develop traditional medicine, connecting it with modern medicine, and studying medicines, herbal medicines, and raw materials, which have been used by people for hundreds of years, and to use them to treat various diseases.1 In every country of the world as well as in our country, viral hepatitis has become widespread. Thus the number of chronic liver diseases, such as liver hardening and liver cancer, is increasing. The main ingredients are those of four herbs, that people have been using in both Tibetan and Mongolian traditional medicine for hundreds of years for liver diseases, namely Saussurea amara L., Salsola collina Pall., Glycyrrhiza uralensis Fisch., Achillea aziatica Serg. The aim of this research is to determine the quality assessment criteria for herbal preparation tablets and to develop pharmacopeia articles. As a result of the study the quality criteria of the tablet were defined, which have completely satisfied the requirements of common tablets. Material and methods: The research was carried out by the Accredited Pharmaceutical Laboratory of the School of Pharmacy and Bio-Medicine. The qualitative and quantitative assessment was measured according to the methods published in the Mongolian National Pharmacopeia /first edition/ and CP 2005, Vol. 1. The quantitative analysis of the amount of glycyrrhizic acid was determined by HPLC. Using HPLC, applications were performed on an Octadecylsilaine, С18 (5 m, 4.0 mm, 250 nm); mobile phase acetic acid-methanol-0.2 mol/L ammonium acetate (13367); detection wavelength 250nm flow rate 0.8 ml/min; column temperature 220 C. 2 Results: Identification and quantification of some of the biological active components of preparation, including glycyrrhizic acid components, were determined, using HPLC, and it was established that glycyrrhizic acid 18.3mg %±0.04. Key words Saussurea Amara L., Achillea asiatica Serg., Salsola Collina Pall., Glycyrrhiza Uralensis Fisch.