Expression of transforming growth factor-beta in cultured normal human lens epithelia cells / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 289-91, 2004.
Article
in English
| WPRIM
| ID: wpr-634155
ABSTRACT
In order to investigate whether cultured normal human lens epithelial cells (LEC) express transforming growth factor beta (TGF-beta), reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical methods were used for detection of TGF-beta mRNA and protein in cultured normal human LEC. The results showed that a single RT-PCR amplified product about 310bp was obtained, and the sequence was homologous to the known sequence. TGF-beta immunostain was positive in the plasma of LEC. It was suggested that normal human LEC could produce TGF-beta, and LEC could be affected by TGF-beta through autocrine action.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Cells, Cultured
/
Transforming Growth Factor beta
/
Epithelial Cells
/
Lens, Crystalline
Language:
English
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2004
Type:
Article
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