Expression of cyclooxygenase-2 mRNA and identification of its splice variant in human myometrium obtained from women in labor / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 5-7, 2005.
Article
in English
| WPRIM
| ID: wpr-634216
ABSTRACT
In order to investigate the expression of cyclooxygenase-2 (COX-2) in human lower segments of myometrium obtained from women in labor and those not in labor and identify the splicing variant of COX-2, reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of COX-2. The primers were designed and synthesized according to the sequence of rat COX-2 splice variant which was discovered firstly by us. Then the splicing variant of COX-2 in human myometrium from woman in labor was identified, cloned into vector and sequenced. The results showed that the expression of COX-2 mRNA was lower in human myometrium obtained from women who were not in labor than that in labor women and a new band of COX-2 was obtained in myometrium from labor woman. The fragment included an unspliced intron, which pitched between exons 7 and 8. It was suggested that COX-2 gene was not only expressed highly in human myometrium from woman in labor, but also produced splicing variant by alternative splicing.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Labor Onset
/
RNA, Messenger
/
Molecular Sequence Data
/
Base Sequence
/
Amino Acid Sequence
/
Sequence Analysis
/
Cyclooxygenase 2
/
Myometrium
Type of study:
Diagnostic study
Language:
English
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2005
Type:
Article
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