Standard and quantitative analysis of cyclin E threshold by cyclin E/DNA multiparameter flow cytometry / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 282-4, 2005.
Article
in English
| WPRIM
| ID: wpr-634268
ABSTRACT
The threshold of cyclin E expression at G1/S boundary is a characteristic feature of cell cycle progressing. In this study, we tried to develop a quantitative approach to analyze cyclin E threshold by multiparameter flow cytometry. The expression of cyclin E in exponentially growing MOLT-4 cells was detected under different photomultiplier tube (PMT) voltages by cyclin E/DNA multiparameter flow cytometry. Additionally, cyclin E was detected in cells which were treated with caffeine and cycloheximide (CHX) under the same PMT voltage. Moreover, the expression of cyclin E in MOLT-4 cells was compared with that in JURKAT cells. Cyclin E threshold was quantified by formula B2/AxC (A, B, C indicates the minimum, threshold, and maximum of cyclin E fluorescence intensity, respectively). Results showed that in MOLT-4 cells, cyclin E threshold calculated by formula B2/AxC was invariable under different PMT settings. It was decreased in cells treated with caffeine and remained changeless in cells treated with cycloheximide. Cyclin E threshold in JURKAT cells was much lower than that in MOLT-4 cells. It was suggested that Formula B2/AxC we firstly set up could be used to analyze cyclin E expression threshold quantitatively.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Caffeine
/
DNA, Neoplasm
/
Leukemia, Lymphoid
/
Cell Cycle
/
Jurkat Cells
/
Cyclin E
/
Cycloheximide
/
Cell Line, Tumor
/
Flow Cytometry
Language:
English
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS