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Development of Species-Specific PCR Primers for the Detection of Streptococcus sobrinus
International Journal of Oral Biology ; : 21-25, 2010.
Article in Korean | WPRIM | ID: wpr-63434
ABSTRACT
This study was undertaken to develop species-specific forward and universal reverse PCR primers for the detection of Streptococcus sobrinus. These primers target the variable regions of the 16S ribosomal RNA coding gene (rDNA) and their specificity was tested against 10 strains of S. sobrinus strains and 20 different species of oral bacteria using serial dilutions of the purified genomic DNA of S.sobrinus ATCC 33478T . Our data show that species-specific amplicons were obtained from all the S. sobrinus strains tested but not from other species. Both direct and nested PCR could detect as little as 400 pg and 4 fg of genomic DNA from S. sobrinus ATCC 33478T , respectively. This result suggests that these PCR primers are highly specific and sensitive and applicable to the detection of S. sobrinus.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Streptococcus / Bacteria / DNA / RNA, Ribosomal, 16S / Polymerase Chain Reaction / Sensitivity and Specificity / Streptococcus sobrinus / Collodion / Clinical Coding Type of study: Diagnostic study Language: Korean Journal: International Journal of Oral Biology Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Streptococcus / Bacteria / DNA / RNA, Ribosomal, 16S / Polymerase Chain Reaction / Sensitivity and Specificity / Streptococcus sobrinus / Collodion / Clinical Coding Type of study: Diagnostic study Language: Korean Journal: International Journal of Oral Biology Year: 2010 Type: Article