Effects of 5-fluorouracil on culture of Bovine Retinal Pigment Epithelial cells
Journal of the Korean Ophthalmological Society
;
: 466-472, 1995.
Article
in Korean
| WPRIM
| ID: wpr-63458
ABSTRACT
Inspite of technical advances, long term retinal reattachment of proliferative vitreoretinopathy following the successful surgery is often disturbed by the occurrence of cell proliferation and traction. A variety of antimetabolite or anticancer drugs have been tried to use for testing their antiproliferative effects on proliferative vitreoretinopathy. To evaluate the antiproliferative property of 5-FU, we treated with the different concentration of 5-FU(0.5, 1, 5, 10 mg/L) on cultured bovine retinal pigment epithelial cells and performed dose inhibition studies. We also examined the 5-FU treated bovine retinal pigment epithelial cells by inverted microscope daily to evaluate the morphologic change for 5 days. The results were as follows; 1. 5-FU inhibited proliferation of bovine pigment epithelial cells in a dose dependent and a time dependent manner. 2. The drug concentration required for 50% inhibition of cell growth(ID50) was found to be 1.87 mg/L. 3. As the drug concentration increased, the cell density gradually decreased and the degenerative changes such as irregular shape of cells, cytoplasmic vaculoes and cell debris were aggravated. In 10 mg/L of 5-FU treated well, disruption of cell membrane and cell death were noted from the first day of incubation. These results show that 5-FU has a potent antiprolifertive effects in retinal pigment epithelial cell and toxic effet in high dose.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Retinaldehyde
/
Traction
/
Cell Count
/
Cell Membrane
/
Cell Death
/
Vitreoretinopathy, Proliferative
/
Cytoplasm
/
Cell Proliferation
/
Epithelial Cells
/
Fluorouracil
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
1995
Type:
Article
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