Differentiation of mesenchymal stem cells into nucleus pulposus cells in vitro / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 156-8, 2008.
Article
in English
| WPRIM
| ID: wpr-634593
ABSTRACT
To find a new source of seed cells for constructing tissue-engineered intervertebral disc, nucleus pulposus (NP) cells and mesenchymal stem cells (MSCs) were isolated from New Zealand white rabbits. The nucleus pulposus cells population was fluorescence-laelled and co-cultured with MSCs with or without direct contact. Morphological changes were observed every 12 h. Semi-quantitative reverse transcriptase-polymerase chain reaction was performed to assess the expression levels of Sox-9, aggreacan and type II collagen every 24 h after the co-culture. MSCs treated with direct contact rounded up and presented a ring-like appearance. The expression of marker genes was significantly increased when cells were co-cultured with direct contact for 24 h. No significant change was found after coculture without direct contact. Co-culture of NP cells and MSCs with direct contact is a reliable method for generating large amount of NP cells used for cell-based tissue engineering therapy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transcription Factors
/
High Mobility Group Proteins
/
Gene Expression
/
Cell Differentiation
/
Cells, Cultured
/
Gene Expression Regulation
/
Collagen
/
Coculture Techniques
/
Reverse Transcriptase Polymerase Chain Reaction
/
Tissue Engineering
Type of study:
Prognostic study
Language:
English
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2008
Type:
Article
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