Stable surface expression of a gene for Helicobacter pylori toxic porin protein with pBAD expression system / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 435-8, 2009.
Article
in English
| WPRIM
| ID: wpr-634744
ABSTRACT
Helicobacter pylori (H. pylori) infection causes peptic and duodenal diseases in humans. Among a 32-protein family of outer membrane proteins, a porin-like protein, HopE, has been a subject of note, mainly for its conservative nature among H. pylori, and for its potential as a vaccine candidate. To achieve stable surface expression of this host cell-toxic protein, hopE gene was introduced into pBAD expression system. After induction with arabinose, all 15 randomly-chosen E. coli LMG 194 colonies from 3 successive passages could express HopE protein, while only 1 from 5 E. coli colonies that contained lac operon-regulated plasmid encoding hopE gene could express HopE. Indirect immunofluorescence confirmed the expression of HopE on E. coli cell surface.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
English
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2009
Type:
Article
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