Investigation of Early Protein Changes in the Urinary Bladder Following Partial Bladder Outlet Obstruction by Proteomic Approach
Journal of Korean Medical Science
;
: 1000-1005, 2005.
Article
in English
| WPRIM
| ID: wpr-63478
ABSTRACT
We investigated the pathophysiological mechanism by proteomic approach as a possible tool to detect the marker proteins to develop lower urinary tract symptoms following bladder outlet obstruction (BOO). Rats were randomized into 3 groups; control, sham operation and BOO groups. BOO group was divided into 1, 3, and 5 day-group. Conventional proteomics was performed with high resolution 2-D gel electrophoresis followed by computational image analysis and protein identification using mass spectrometry using rat urinary bladders. A comparison of bladder of BOO group with control bladder showed that three proteins of optineurin, thioredoxin and preprohaptoglobin were over-expressed in the bladder of BOO group. In addition, four proteins, such as peroxiredoxin 2, transgelin, hippocampal cholinergic neurostimulating peptide (HCNP) and beta-galactoside-binding lectin, were under-expressed in the bladder of BOO group. These data supported that downregulation of HCNP might make detrusor muscle be supersensitive to acetylcholine, up-regulation of optineurin means the protection of nerve injury, and down-regulation of transgelin means the decreased contractility of detrusor muscle. Beside these proteins, other proteins are related to oxidative stress or have a nonspecific function in this study. However more information is needed in human bladder tissue for clinical usage.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Urinary Bladder
/
Urinary Bladder Neck Obstruction
/
Electrophoresis, Gel, Two-Dimensional
/
Proteins
/
Down-Regulation
/
Gene Expression
/
Up-Regulation
/
Rats, Sprague-Dawley
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Proteomics
Limits:
Animals
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2005
Type:
Article
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