Spectrophotometric determination of trimethylamine-nitrogen in cadaver tissues for the estimation of late postmortem interval: a pilot study / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 630-3, 2008.
Article
in English
| WPRIM
| ID: wpr-634975
ABSTRACT
To study the relationship between the late postmortem interval (PMI) and trimethylamine-nitrogen (TMA-N) in postmortem tissues of cadaver, TMA-N in muscles, livers and kidneys of rats was measured at different postmortem intervals (PMI) by using a modified spectrophotometric method. The results indicated that the detection sensitivity of TMA-N was 1 mg/L, and there was a good linear correlation between the value of absorbance (A value) and TMA-N at the concentration of 1-10 mg/L (R (2) = 0.9991). Although TMA variation in muscles was different from that in inner organs during the time since death, TMA-N changes in cadaver tissues was positively correlated with PMI. During 2 to 7 d since death, the best correlation between PMI and TMA-N concentration was found in muscles. With PMI as an independent variable, the cubic polynomial regression equation was y= -0.457x(3)+6.519x(2)-24.574x+27.207 (R (2)=0.969). During 3 to 8 days since death, PMI was best correlated with TMA-N concentration in inner organs. With PMI as the independent variable, the cubic polynomial regression equation was y=0.509x(3)-9.153x(2)+55.727x-95.819 (R (2)=0.953). It was concluded that TMA-N in tissues could be used as a new estimator for late PMI. The method used in this study offered advantages such as accuracy, sensitivity, little samples required and wide PMI estimation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Postmortem Changes
/
Spectrophotometry
/
Time Factors
/
Cadaver
/
Random Allocation
/
Pilot Projects
/
Rats, Sprague-Dawley
/
Forensic Pathology
/
Methylamines
/
Nitrogen
Type of study:
Controlled clinical trial
Language:
English
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2008
Type:
Article
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