Effects of gamma-irradiation on intracellular proliferation of Toxoplasma gondii RH tachyzoites
The Korean Journal of Parasitology
;
: 173-178, 1995.
Article
in English
| WPRIM
| ID: wpr-63515
ABSTRACT
A quantitative assay was performed on the effects of gamma-irradiation (30-300 Gy) on intracellular proliferation of Toxoplasma gondii RH tachyzoites in human leukemic HL-60 cells and murine peritoneal macrophages by means of 3H-uracil uptake assay. Infected non- irradiation group (NI) and uninfected group (incubating only host cells) were prepared. The 3H-uracil uptake by tachyzoites of NI group 12-24 hrs after infection was 2,190-4,787 counts per minute for macrophages and 2,967-8,254 for HL-60 cells, whereas the irradiated tachyzoites revealed only 381-703 (100 Gy) and 218-408 (300 Gy) for macrophages, and 1,911-2,618 (30 Gy), 1,253-1,384 (70 Gy), 1,013-1,090 (100 Gy), and 483-588 (300 Gy) for HL-60 cells. The proliferation inhibition rate was similar in macrophages and HL-60 cells, for example, 89-94% and 80-94% respectively by 300 Gy, 12-24 hrs after infection. It is concluded that RH tachyzoites of T. gondii are severely affected by gamma-irradiation in their capability of intracellular proliferation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Radiation Dosage
/
Toxoplasma
/
Cell Division
/
Cells, Cultured
/
HL-60 Cells
/
Gamma Rays
/
Animals
/
Macrophages
/
Mice, Inbred BALB C
/
Mice, Inbred ICR
Limits:
Animals
/
Humans
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
1995
Type:
Article
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