Expression in E.Coli and Antigenicity of HGV NS5 Protein / 中国病毒学
Virologica Sinica
;
(4): 114-118, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-635212
ABSTRACT
A 880 bp cDNA localized to the putative NS5 region of HGV genome was expressed in E.coli BL21(DE3). The cDNA fragment was inserted into a plasmid pGEX-5X-1,at the downstream of the DNA sequence encoding Schistosoma japonicum glutathione S- transferase(GST),in the same reading frame with the gene of GST. A 60KD GST-NS53 fusion protein was expressed at 37℃ in a form of inclusion bodies amounting to 30 percent of total host protein whereas at 20℃ mainly in a form of soluble protein . The fusion protein was extracted and purified to homologue. The purified GST-NS53 fusion protein could be specifically recognized with either the sera from the patient infected by HGV or the antisera directed against GST.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Virologica Sinica
Year:
2001
Type:
Article
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