Adenylate cyclase stimulation and ocular hypertension inhibition by forskolin analogs / 中华实验眼科杂志
Chinese Journal of Experimental Ophthalmology
;
(12): 1-4, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-635279
ABSTRACT
ObjectiveForskolin (FSK) analogs,isoforskolin (isoF),deacetylforskolin(deaF),and 1-acetylforskolin(1-aF),extracted from Coleus forskohlii native to Yunnan,were assayed for their adenylate cyclase stimulating activities in vitro and for effects of two analogs on ocular hypertension (OHT) in water-loaded rabbits.MethodsAdenylate cyclase stimulation was determined by protein-binding method of radioimmunoassay,and intraocular pressure was monitored by pneumatonometer.ResultsIt showed that isoforskolin and forskolin stimulated adenylate cyclase in vitro with almost equal activity,deacetylforskolin with milder activity,and 1-acetylforskolin with little activity in vitro.1% deaF and 1-aF suppressed rabbit OHT induced by water-loading for at least 3h,with the maximal inhibitory rates of 6.0,10.9% respectively.ConclusionThis study suggests that two foskolin analogs (isoforskolin,deacetylforskolin) possess adenylate cyclase stimulation activities in vitro;deacetylforskolin and 1-acetylforskolin suppress OHT induced by water-loading in rabbits.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Experimental Ophthalmology
Year:
2001
Type:
Article
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