Expressions of endoplasmic reticulum stress related protein, hypoxia-inducible factor-1αand vascular endothelial growth factor in the retina of diabetic rats / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
;
(12): 772-776, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-637360
ABSTRACT
?AlM To evaluate the expressions and significances of endoplasmic reticulum stress related protein ( BlP ) , hypoxia-inducible factor-1α ( HlF-1α) and vascular endothelial growth factor ( VEGF) in the retina of diabetic rats. ?METHODS:
Seventy-two male Sprague-Dawley ( SD) rats were chosen and divided randomly into 6 groupsnormal control 2mo (C2m, n = 12), diabetes mellitus 2mo (D2m, n=12), normal control 4mo (C4m, n=12), diabetes mellitus 4mo (D4m, n = 12), normal control 6mo (C6m, n=12) and diabetes mellitus 6mo (D6m, n=12 ) . The diabetes mellitus mouse were induced by intraperitoneal injection of 0. 1mol/L streptozotocin (STZ, 65mg/kg). The expression of BlP, HlF-1α and VEGF in the retina were detected by enzyme- linked immuno sorbent assay. The location of BlP, HlF-1α and VEGF in epiretinal membranes were investigated by immunohistochemistry staining. ?RESULTS:
BlP were significantly increase than control groups in all DM groups with the course of diabetes ( P ?CONCLUSlONThe expressions of BlP, HlF-1α and VEGF increase in the retina of diabetic rats than control groups with progressive of diabetes mellitus, both endoplasmic reticulum stress and HlF-1α signal path play important role in the progress of diabetic retinopathy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
International Eye Science
Year:
2015
Type:
Article
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