Changes of brain - derived neurotrophic factor expression in gene modified bone marrow mesenchymal stem cells / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
;
(12): 1816-1819, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-637949
ABSTRACT
AIM:
To study the changes of brain - derived neurotrophic factor ( BDNF ) expression in gene modified bone marrow mesenchymal stem cells ( BMSC) . ●METHODS:
BMSC were divided into blank control group ( without transfected BMSC ) , negative control group ( empty vector without BDNF gene transfected BMSC) and experimental group ( BDNF gene transfected BMSC) . The expression of BDNF mRNA in BMSC was measured by Realtime PCR, and the expression of BDNF in BMSC was measured by ELlSA. ●RESULTS:
The BDNF mRNA expressions of 3, 4, 5, 6, 7 and 8-generation BMSC cells in the experimental group were higher than those in the blank control group and negative control group. The differences were statistically significant (P3 F=491. 788, P ●CONCLUSION:
Long-term expression of BDNF in BMSC can be enhanced by genetic engineering.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
International Eye Science
Year:
2016
Type:
Article
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