Development of porcine respiratory and reproductive syndrome virus replicon vector for foot-and-mouth disease vaccine
Clinical and Experimental Vaccine Research
;
: 100-109, 2014.
Article
in English
| WPRIM
| ID: wpr-63816
ABSTRACT
PURPOSE:
Foot-and-mouth disease (FMD) is an economically important global animal disease. To control FMD virus (FMDV) outbreaks, a lot of different novel approaches have been attempted. In this study, we proposed a novel porcine reproductive and respiratory syndrome virus (PRRSV) as a replicon vector to express FMDV structural protein. MATERIALS ANDMETHODS:
PRRSV infectious clone (PRRSVK418DM) was used to develop an expression vector through the reverse genetic manipulation of PRRSV; FMDVP12A3C gene of serotype O was synthesized and used for an antigen. MARC-145 cells (African green monkey kidney epithelial cell line) were used for electroporation mediated transfection. The transfection or the expression of P12A3C and N protein of PRRSV was analyzed by either replicon containing PRRSV alone or by co-infection of helper PRRSV.RESULTS:
We constructed PRRSVK418DM replicon vector containing FMDVP12A3C, and genome sequences were confirmed by subsequent sequence analysis. In vitro expression of P12A3C and PRRSV N protein was confirmed by immunofluorescence antibody assay using antibodies specific for PRRSV N protein (anti-PRRSV N MAb), FMDV-VP1 (anti-VP1 MAb).CONCLUSION:
The results indicate that PRRSV replicon vector can be a promising novel vector system to control FMDV and useful for vaccine development in the future.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Replicon
/
Viruses
/
Transfection
/
Chlorocebus aethiops
/
Disease Outbreaks
/
Fluorescent Antibody Technique
/
Genome
/
Clone Cells
/
Sequence Analysis
/
Electroporation
Limits:
Animals
Language:
English
Journal:
Clinical and Experimental Vaccine Research
Year:
2014
Type:
Article
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