Effect of Myriocin on Hypertrophy of Glomerular Mesangial Cells and Extracellular Matrix Production Induced by High Glucose / 实用儿科临床杂志
Journal of Applied Clinical Pediatrics
;
(24)1992.
Article
in Chinese
| WPRIM
| ID: wpr-638823
ABSTRACT
Objective To observe the changes of extracellular matrix(ECM) production and hypertrophy of glomerular mesangial cells(GMCs) induced by high glucose(HG),and the inhibitory role of myriocin(ISP-1).Methods GMC cultured with normal glucose(5.6 mmol/L D-Glucose,NG),HG(25.2 mmol/L D-Glucose) and HG plus ISP-1(100 mg/L) for different durations(0,24,48,(72 h)).The sizes of GMC were indicated by forward scatter intensity,measured by flow cytometery,and the levels of fibronection(FN),collagen Ⅳ(Col Ⅳ),laminin(LN),precollagen Ⅲ(Pcol Ⅲ) and hyaluronic acid(HA) in the supernatant of cultured GMC were detec-(ted) by ELISA.Results Compared with NG,HG could induce GMC hypertrophy(P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Applied Clinical Pediatrics
Year:
1992
Type:
Article
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